The non-communicable diseases (NCDs) are one of the leading causes of death globally. Around 82% of the premature deaths due to NCDs occur in the low-and middle-income countries. The study aimed to assess the knowledge of dietary habits and behaviour-related determinants of NCD in urban Nepalese women of Eastern Nepal. A cross-sectional study was designed by using interviewer-administered questionnaire regarding knowledge on NCD. The definitions used for the study adopted the WHO STEP wise approach to chronic disease risk factor surveillance (STEPS) survey. For the socioeconomic status, modification of Kuppuswamy Socioeconomic Status Scale in context to Nepal was used and the ethnic groups were classified according to the National Central Bureau of Statistics of Nepal. A total of 706 women of one municipality aged 20â€“59 years were randomly selected. The knowledge scores was 62.14% Â± 14.93% . The mean score according to domains were diet- and behaviour-related causes (75.25%), diet quality (45.27%) fruit and vegetable link (30.02%), health consequences of obesity (76.82%), causes of cardiovascular disease (77.08%) and causes of certain cancers (36.10%). The best understood domain was found in causes of cardiovascular disease (CVD). The total scores of knowledge regarding NCD were found to be significant with caste/ethnicity, education level, occupation, socioeconomic status, physical activity and fruit intake. There was poor knowledge of the benefit of eating fruit and vegetables for NCD and other preventable causes of certain cancers. Education targeting the benefits of vegetables and fruit may have the greatest impact on NCD prevention. Key word: Non communicable disease, dietary habit, WHO STEP questionnaire Key messages: 1.There was poor knowledge of the benefit of eating fruit and vegetables and other preventable causes of certain cancers. 2.Nutrition education messages need to be communicated within the general population 3.Education targeting the benefits of vegetables and fruit may have the greatest impact on NCD prevention.